A photoreceptor cell
with green synaptic vesicles. |
The Laboratory of Neuroendocrinology - Molecular Cell
Physiology is part of the
Institute of
Pathophysiology,
Medical Faculty, University of Ljubljana,
Slovenia.
The longterm program of the Laboratory of
Neuroendocrinology, is to study molecular mechanisms of
regulated exocytotic hormone secretion. The function of
a single cell is studied by electrophysiological
techniques of patch-clamping, where the secretory
activity is monitored by changes in membrane
capacitance. Since cytosolic calcium activity is the
trigger of exocytosis in excitable cells, fluorescence
(photometric and imaging) methods are used to measure
changes in cytosolic calcium. Caged calcium photolysis
is used to produce a step change in cytosolic calcium. A
method was developed to quantify the interaction
between a single secretory granule and plasmalemma,
detected as discrete steps in membrane capacitance
(femtofarad steps). A combination of molecular biology
and electrophysiology approach was adopted to inhibit
expression of monomeric GTP-binding proteins. Confocal
microscopy is used to study molecular mechanisms of
regulated exocytosis in endocrine cells, astrocytes and
photoreceptors. We also use confocal microscopy to
evaluate our bioengeneering products.
Links to
recent meetings organized by
LN-MCP:
- International
Conference on Brain Energy Metabolism - ICBEM 2024
-
Predavanje: prof. dr.
Mark Tester, 11. 4. 2016
- Workshop ASTROGLIA
2014, Ljubljana, July 10-12
- IBRO GLIA Workshop:
Membrane capacitance techniques to study regulated
exocytosis, San Juan-Bayamon, Puerto Rico, 2013
-
ISN Satellite meeting
- Glial cells in (patho)physiology2011
-
Cell Sciences Seminar & Super-Resolution
Microscopy Workshop 2010
-
FEPS 2009 – The physiology meeting organized by The
Slovenian Physiological Society, The Austrian
Physiological Society, and The Federation of
European Physiological Societies.
-
International
Meeting "Mechanism(s) of Exocytosis 2008"
and "15th Young Neuroscientists Meeting"
-
International
Cell Physiology & Engineering Meeting;
12th Young Neuroscientists Meeting;
Advanced Microscopy Workshop 2005;
LN-MCP is a partner of Edu-GLIA
Edu-GLIA is an
Initial Training Network (ITN) funded by the
European Commission under the Seventh Framework
Programme (FP7).
Bibliography |
