Work in the clean room.

Techniques

Single channel recording
Whole-cell patch-clamp recording
Patch-clamp amplifier development and design
Two microelectrode voltage-clamp
Cell membrane capacitance measurements using a two-phase lock-in amplifier
- Uncompensated computer aided reconstruction of passive electrical cell parameters
- Compensated high resolution measurements of cell membrane capacitance changes
Fluorescence-based measurements of intracellular ion activities
- Dual/single excitation - dual/single emmision photometry
- Dual/single excitation - single emmision imaging Flash photolysis
Various microinjection methods
- Modulation of protein expression using antisense oligodeoxynucleotides
- Modulation of secretory activity with synthetic oligopeptides
- Modulation of secretory activity by microinjection of recombinant proteins
Cell culture laboratory
Biochemical cell population secretion studies
Immunocytochemistry
Amperometry, cyclic voltametry
Confocal microscopy
Image processing - deconvolution
Image analysis
Software design
Techniques in molecular biology
- Molecular cloning and DNA analysis
- RT-PCR & single cell RT-PCR
Techniques in biochemistry
- Overexpression and protein purification
- Chromatographic techniques
- Immunoblotting
- Immunoprecipitation
- in vitro transcription/translation
- subcellular fractionation